How Does Indole-3-Butyric Acid Promote Rooting and Enhance Plant Propagation?

2025-08-13 10:17

Technical Introduction

Indole-3-Butyric Acid (IBA, CAS No. 133-32-4) is a synthetic auxin plant growth regulator widely used to stimulate root formation in plant cuttings and tissue culture.
It structurally resembles the natural auxin indole-3-acetic acid (IAA) but is more stable and effective in promoting adventitious root development.
IBA promotes cell elongation and differentiation in root primordia, improving rooting success and uniformity.


Applications

1. Agriculture and Horticulture

  • Rooting Agent: Widely used in propagation of ornamentals, fruit trees, and forestry species to induce rooting in cuttings.

  • Seedling Development: Enhances root growth and establishment in seedlings and transplants.

2. Tissue Culture

  • Promotes root initiation in micropropagation protocols across a broad range of plant species.


Advantages

  • High Efficiency: Effective at low concentrations for rooting stimulation.

  • Chemical Stability: More resistant to degradation than natural auxins, ensuring consistent results.

  • Versatility: Suitable for use in powders, gels, dips, and liquid formulations.


Typical Parameters

PropertyValue
Molecular FormulaC₁₂H₁₃NO₂
Molecular Weight203.24 g/mol
AppearanceWhite crystalline powder
Purity (%)≥98.0 (technical grade)
SolubilitySlightly soluble in water; soluble in alcohols and organic solvents
StorageStore in a cool, dry, and dark place
Shelf Life24–36 months

FAQs

Q1: How is IBA applied for rooting?
A1: Typically as a powder or solution dip on cuttings before planting; concentrations vary by species.

Q2: Is IBA safe for edible crops?
A2: Yes, when used as directed; residue levels are minimal and regulated.

Q3: Can IBA be mixed with other PGRs?
A3: Yes, often combined with cytokinins like 6-BA for balanced shoot and root growth.

Q4: Does IBA replace fertilizers?
A4: No, it regulates growth but does not provide nutrients.

Q5: How soon does rooting occur after IBA treatment?
A5: Root initiation can be seen within 7–14 days, depending on species and conditions.

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