Introduction to Thidiazuron
Thidiazuron (TDZ) is a plant growth regulator primarily used for its cytokinin-like effects. It plays a critical role in stimulating cell division, delaying senescence, and promoting defoliation in crops. Though it was originally developed as a cotton defoliant, its use has expanded to horticulture and tissue culture due to its high efficacy in regulating plant growth responses.
Chemical Properties and Mechanism of Action
Thidiazuron has the chemical formula C₉H₈N₄OS and belongs to the phenylurea class of compounds. Its function mimics natural cytokinins by enhancing cell division and shoot proliferation. Unlike typical cytokinins, TDZ is not easily broken down in plants, giving it long-lasting activity. It interferes with the plant's hormonal balance, especially auxin and cytokinin pathways, to trigger physiological changes such as leaf abscission and bud induction.
Agricultural and Horticultural Applications
In cotton farming, TDZ is used to induce defoliation before mechanical harvesting, improving fiber quality and reducing leaf contamination. In fruit crops like apples and grapes, it enhances fruit size and improves uniform ripening. In plant tissue culture, TDZ is valued for inducing shoot regeneration and somatic embryogenesis, especially in woody plants and recalcitrant species.
Key Benefits of Thidiazuron
Effective defoliation: Ensures clean cotton harvesting with minimal damage.
Improved fruit quality: Enhances size, color, and uniformity.
Stimulates regeneration: Widely used in micropropagation and callus induction.
Long-lasting action: Remains active in plant systems longer than natural cytokinins.
Low usage rate: Effective at low concentrations, making it cost-efficient.
Limitations and Considerations
TDZ is highly potent, and improper use can lead to phytotoxic effects such as leaf burn or excessive callus formation. Timing and dosage are critical. Its persistence also means it may interfere with other plant growth stages if not properly managed. In some markets, its regulatory approval is limited, so growers must check local guidelines.
Environmental and Safety Aspects
Thidiazuron is generally considered safe when used according to recommended practices. It has low toxicity to humans and animals, but care should be taken to avoid unnecessary exposure. Users should wear protective clothing and avoid drift to non-target crops or natural vegetation. It degrades in soil over time and poses minimal risk of bioaccumulation.
Formulations and Best Practices
TDZ is available in suspension concentrates and soluble powder forms. In cotton, it is usually applied 1–2 weeks before harvest. In tissue culture, it is added to media in microgram-level concentrations. For optimal results, environmental factors like temperature and crop maturity should be considered when planning applications.
FAQ About Thidiazuron
Q1: What is the primary use of Thidiazuron in agriculture?
It is mainly used as a cotton defoliant to promote leaf drop before harvest and also to improve fruit characteristics in horticulture.
Q2: Is Thidiazuron a cytokinin?
Not exactly. It is not a natural cytokinin but has strong cytokinin-like activity, which makes it functionally similar in plant systems.
Q3: Can it be used in all crops?
No. Its use is crop-specific and should be guided by local regulations and research data. It's commonly used in cotton, apples, grapes, and ornamental plant tissue culture.
Q4: What are the risks of using too much TDZ?
Overuse can cause leaf necrosis, abnormal growth, or failure in normal fruit or flower development due to hormonal imbalance.
Q5: Is Thidiazuron safe for humans and the environment?
Yes, when applied correctly. It has low toxicity and degrades in soil over time, but handlers should follow safety instructions and wear protection during application.